Human Coronary Artery Smooth Muscle Cel

Code: 350-05A D2-231

Application

vascular research, supply of blood to heart muscle

Cell Line Origin

Artery

Components

Basal Medium conta...


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Your Price
€1,140.00 EACH
€1,402.20 inc. VAT

Application

vascular research, supply of blood to heart muscle

Cell Line Origin

Artery

Components

Basal Medium containing 10% FBS & 10% DMSO

General description

Human Coronary Artery Smooth Muscle Cells (HCASMC) provide an excellent model system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis.HCASMC has been utilized in a number of research studies, for example, to: Study signaling pathways regulating smooth muscle differentiation (Zhou, 2010); and chronic inflammation of arterial wall that leads to artherosclerosis (Kiyan, 2014) Demonstrate that STAT-1 and STAT-3 regulate VEGF production in smooth muscle cells by having opposing effects on HIF-1α expression (Albasanz-Puig, 2012); study the mechanisms of hypoxia and reoxigenation injuries in by demonstrating increased production of ROS and inflammatory cytokines, and further showing that DHA is not beneficial in this type of injuries (Feng, 2012) Investigate the gene expression differences between smooth muscle cells from different arteries, underlying their differential response to injuries and proliferation stimuli (Lange, 2013) Suggest the hypermethylation of SOCS3 gene as the connection between TNF-α and IGF-1 released in response to mechanical injury during coronary intervention, and the induction of cytokines leading to intimal hyperplasia and restenosis (Dhar, 2013) Develop a novel VEGFR/MET-targeted inhibitor with improved antitumor efficacy and decreased toxicity (Fujita, 2013); and investigate novel therapies and drug combinations to achieve optimal target selectivity (Lehar, 2009; Wo-Wong, 2013) Develop elastic scaffolds for tissue engineering (Nivison-Smith, 2010, 2012) and novel treatment strategies to prevent stent restenosis by designing new materials (Crowder, 2012), or drug therapies to preferentially inhibit smooth muscle cell growth (O’;Neill, 2009; Mociornita, 2013)Characterization: positive for smooth muscle cell specific alpha-actin expression.

Preparation Note

2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO Can be cultured at least 16 doublings

Subculture Routine

Please refer to the HCASMC Culture Protocol.

biological sourcehuman coronary artery (normal, tunica intima and media)
growth modeAdherent
karyotype2n = 46
manufacturer/tradenameCell Applications, Inc
morphologysmooth muscle
packagingpkg of 500,000 cells
Quality Level100
relevant disease(s)diabetes; stroke; cardiovascular diseases
shipped indry ice
storage temp.−196°C
technique(s)cell culture | mammalian: suitable
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